Western blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Product Name | Rabbit anti-RASGRP4 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | DogHumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RASGRP4 |
|---|---|
| Gene Synonyms | - |
| Gene Full Name | RAS guanyl releasing protein 4 |
| Gene Summary | The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Molecular Weight(MW) | 63kDa |
| Sequence | 576 amino acids |
| Entrez Gene | 115727 |
|---|---|
| SwissProt ID | C0LTP7 |
| Protein Accession | NP_001139679 |
Western blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
