Immunoblot
Lane1: 30 ug human placental tissue lysateLane2: 30 ug human placental tissue lysateLane3: 30 ug human placental tissue lysateLane4: 30 ug human placental tissue lysateLane5: 20 ug human myometrial tissue lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Goat anti-rabbit HRPSecondary Antibody Dilution: 1:10000Gene Name: Nitric oxide synthase 2, inducible (NOS2)Submitted by: Hiten Mistry, Ania Czajka and Marta Hentschke Ribeiro, King's College LondonWestern blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Product Name | Rabbit anti-NOS2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HEP-NOS, INOS, NOS, NOS2A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanPig |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOS2 |
|---|---|
| Gene Synonyms | HEP-NOS INOS NOS NOS2A |
| Gene Full Name | Nitric oxide synthase 2, inducible |
| Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. |
| Alternative Names | HEP-NOS INOS NOS NOS2A |
| Molecular Weight(MW) | 110kDa |
| Sequence | 997 amino acids |
Immunoblot
Lane1: 30 ug human placental tissue lysateLane2: 30 ug human placental tissue lysateLane3: 30 ug human placental tissue lysateLane4: 30 ug human placental tissue lysateLane5: 20 ug human myometrial tissue lysatePrimary Antibody Dilution: 1:500Secondary Antibody: Goat anti-rabbit HRPSecondary Antibody Dilution: 1:10000Gene Name: Nitric oxide synthase 2, inducible (NOS2)Submitted by: Hiten Mistry, Ania Czajka and Marta Hentschke Ribeiro, King's College London
Western blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM119395