Immunoblot
Lane1: 40 ug human HCT116 cell lysateLane2: 40 ug 1mM hydroxyurea treated HCT116 cell lysateLane3: 40 ug 10uM Etoposide treated human HCT116 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: CCNB2Submitted by: Claudia Schafer & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University JenaWestern blot
Titration: 1.0 ug/mlPositive Control: NCI-H226 Whole Cell| Product Name | Rabbit anti-CCNB2 polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HsT17299 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRabbitRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCNB2 |
|---|---|
| Gene Synonyms | HsT17299 |
| Gene Full Name | Cyclin B2 |
| Gene Summary | Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. |
| Alternative Names | HsT17299 |
| Molecular Weight(MW) | 44kDa |
| Sequence | 398 amino acids |
Immunoblot
Lane1: 40 ug human HCT116 cell lysateLane2: 40 ug 1mM hydroxyurea treated HCT116 cell lysateLane3: 40 ug 10uM Etoposide treated human HCT116 cell lysatePrimary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:5000Gene Name: CCNB2Submitted by: Claudia Schafer & Dr. Oliver Kramer, Institute for Biochemistry and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University Jena
Western blot
Titration: 1.0 ug/mlPositive Control: NCI-H226 Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
