Western blot
Titration: 1.0 ug/mlPositive Control: HepG2 Whole Cell| Product Name | Rabbit anti-MCAM polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD146, MUC18 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHorsePigRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MCAM |
|---|---|
| Gene Synonyms | CD146 MUC18 |
| Gene Full Name | Melanoma cell adhesion molecule |
| Gene Summary | MCAM plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. MCAM could be an adhesion molecule active in neural crest cells during embryonic development. MCAM acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration. |
| Alternative Names | CD146 MUC18 |
| Molecular Weight(MW) | 71kDa |
| Sequence | 646 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: HepG2 Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
