Western blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Product Name | Rabbit anti-SLC7A5 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 4F2LC, CD98, D16S469E, E16, LAT1, MPE16, hLAT1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SLC7A5 |
|---|---|
| Gene Synonyms | 4F2LC CD98 D16S469E E16 LAT1 MPE16 hLAT1 |
| Gene Full Name | Solute carrier family 7 (amino acid transporter light chain, L system), member 5 |
| Gene Summary | SLC7A5 is a sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. SLC7A5 is involved in cellular amino acid uptake. SLC7A5 acts as an amino acid exchanger. SLC7A5 is involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. SLC7A5 plays a role in neuronal cell proliferation (neurogenesis) in brain. SLC7A5 is involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. SLC7A5 is involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. SLC7A5 may play an important role in high-grade gliomas. SLC7A5 mediates blood-to-retina L-leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. SLC7A5 acts as the major transporter of tyrosine in fibroblasts. |
| Alternative Names | 4F2LC CD98 D16S469E E16 LAT1 MPE16 hLAT1 |
| Molecular Weight(MW) | 56kDa |
| Sequence | 507 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: Jurkat Whole Cell| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM119647