Western blot
Titration: 1.0 ug/mlPositive Control: A549 Whole CellImmunoblot
1: Maduca sexta intestine lysate-untreated, 2: Maduca sexta intestine lysate-2 hour crytoxin, 3: Maduca sexta intestine lysate-4 hour crytoxin, 4: Maduca sexta intestine lysate-6 hour crytoxin, 5: Maduca sexta intestine lysate-12 hour crytoxin, 6: Maduca sexta intestine lysate-24 hour crytoxin.Primary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:10,000Gene Name: RPS3Submitted by: Dra. Helena Porta Ducoing and Biol. Gladys Jimenez Nopala| Product Name | Rabbit anti-RPS3 polyclonal antibody - middle region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ26283, FLJ27450, MGC87870, S3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMouseZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS3 |
|---|---|
| Gene Synonyms | FLJ26283 FLJ27450 MGC87870 S3 |
| Gene Full Name | Ribosomal protein S3 |
| Gene Summary | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. |
| Alternative Names | FLJ26283 FLJ27450 MGC87870 S3 |
| Molecular Weight(MW) | 27kDa |
| Sequence | 243 amino acids |
Western blot
Titration: 1.0 ug/mlPositive Control: A549 Whole Cell
Immunoblot
1: Maduca sexta intestine lysate-untreated, 2: Maduca sexta intestine lysate-2 hour crytoxin, 3: Maduca sexta intestine lysate-4 hour crytoxin, 4: Maduca sexta intestine lysate-6 hour crytoxin, 5: Maduca sexta intestine lysate-12 hour crytoxin, 6: Maduca sexta intestine lysate-24 hour crytoxin.Primary Antibody Dilution: 1:1000Secondary Antibody: Anti-rabbit HRPSecondary Antibody Dilution: 1:10,000Gene Name: RPS3Submitted by: Dra. Helena Porta Ducoing and Biol. Gladys Jimenez Nopala| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
