Western blot
Positive Control: Lane 1: 60ug human NT2 cell line Lane 2: 80ug mouse brain extractPrimary Antibody Dilution : 1:500Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR BioscienceSecondry Antibody Dilution : 1:20,000Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
human Hela Cells and Monkey VeraImmunoblot
1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: Rab11aSubmitted by: Dr. Yuzhi Chen, University of Arkansas for Medical ScienceWestern blot
RAB11A antibody - C-terminal region validated by WB using Placenta Lysate at 1ug/ml.| Product Name | Rabbit anti-RAB11A polyclonal antibody - C-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC1490, YL8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineHumanMouseZebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAB11A |
|---|---|
| Gene Synonyms | MGC1490 YL8 |
| Gene Full Name | RAB11A, member RAS oncogene family |
| Gene Summary | The protein encoded by this gene belongs to the Rab family of the small GTPase superfamily. It is associated with both constitutive and regulated secretory pathways, and may be involved in protein transport. Two transcript variants encoding different isoforms have been found for this gene. |
| Alternative Names | MGC1490 YL8 |
| Molecular Weight(MW) | 24kDa |
| Sequence | 216 amino acids |
Western blot
Positive Control: Lane 1: 60ug human NT2 cell line Lane 2: 80ug mouse brain extractPrimary Antibody Dilution : 1:500Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR BioscienceSecondry Antibody Dilution : 1:20,000Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
human Hela Cells and Monkey Vera
Immunoblot
1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)Primary Antibody Dilution: 2ug/mlSecondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR BioscienceSecondary Antibody Dilution: 1: 20,000Gene Name: Rab11aSubmitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Western blot
RAB11A antibody - C-terminal region validated by WB using Placenta Lysate at 1ug/ml.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
