Immunoblot
Lane 1: 25ng purified Human Alveolar sample (w/SP-A1+SP-A2) Lane 1: 25ng purified Human Alveolar sample (w/SP-A1+SP-A2)Lane 2: 20ug Rat bronchoalveolar lavageLane 3: 25ng hSP-A2 variant expressed in CHO cellsLane 4: 25ng hSP-A2 variant expressed in CHO cellsLane 5: 25ng hSP-A1/A2 variants expressed in CHO cellsLane 6: 25ng hSP-A1/A2 variants expressed in CHO cellsLane 7: 20ug SP-A1/2 KO mouse bronchoalveolar lavageLane 8: 20ug hSP-A2 variant transgenic mouse bronchoalveolar lavageLane 9: 20ug hSP-A2 variant transgenic mouse bronchoalveolar lavageLane 10: 20ug hSP-A1 variant transgenic mouse bronchoalveolar lavageLane 11: 20ug hSP-A1 variant transgenic mouse bronchoalveolar lavageLane 12: 20ug hSP-A1/A2 variants transgenic mouse bronchoalveolar lavageLane 13: 20ug mSP-A1/A2 bronchoalveolar lavage; +/- mouseLane 14: 20ug mSP-A1/A2 bronchoalveolar lavage; WT mouseLane 15: 10ug human alveolar cell lysateLane 16: 25ng purified hSP-A1/A2Primary Antibody Dilution: 1:2500Secondary Antibody: IgG HRP ConjSecondary Antibody Dilution: 1:10000Gene Name: SFTPA2Submitted by: Todd M. Umstead, Pennsylvania State University College of MedicineImmunoblot
1: 20ng human SP-A2 protein, 2: 20ug rat wt BAL cell lysate, 3: 25ng hSP-A2 protein purified from transfected mouse CHO lysate, 4: 25ng hSP-A2 protein purified from transfected mouse CHO lysate, 5: 25ng hSP-A1 protein purified from transfected mouse CHO lysate, 6: 25ng hSP-A1 protein purified from transfected mouse CHO lysate, 7: 25ng hSP-A1/A2 protein purified from transfected mouse CHO lysate, 8: 25ng hSP-A1/A2 protein purified from transfected mouse CHO lysate, 9: 20ug SP-A KO mouse BAL lysate, 10: 25ng hSP-A2 protein purified from transfected mouse BAL lysate, 11: 25ng human SP-A2 protein purified from transfected mouse BAL lysate, 12: 25ng human SP-A1 protein purified from transfected mouse BAL lysate, 13: 25ng human SP-A1 protein purified from transfected mouse BAL lysate, 14: 25ng human SP-A1/2 protein purified from transfected mouse BAL lysate, 15: 25ng mouse SP-A2 protein purified from mouse BAL lysate, 16: 20ug mouse wt BAL lysate, 17: 15ug human wt T2 lysate, 18: 25ng human SP-A2 protein.Primary Antibody Dilution: 1:2500Secondary Antibody: goat anti-rabbit-HRPSecondary Antibody Dilution: 1:10,000Gene Name: SFTPA2Submitted by: Todd M. Umstead, David S. Phelps, Joanna Floros, Penn State Center for Host defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics, The Pennsylvania State University College of MedicineWestern blot
Titration: 1.0 ug/mlPositive Control: Fetal Heart| Product Name | Rabbit anti-SFTPA2 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COLEC5, FLJ50594, FLJ50597, FLJ51953, FLJ79091, FLJ93678, MGC133169, MGC133366, MGC189714, MGC189761, PSAP, SFTP1, SFTPA2B, SPA2, SPAII |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SFTPA2 |
|---|---|
| Gene Synonyms | COLEC5 FLJ50594 FLJ50597 FLJ51953 FLJ79091 FLJ93678 MGC133169 MGC133366 MGC189714 MGC189761 PSAP SFTP1 SFTPA2B SPA2 SPAII |
| Gene Full Name | Surfactant protein A2 |
| Gene Summary | This gene is one of several genes encoding pulmonary-surfactant associated proteins (SFTPA) located on chromosome 10. Mutations in this gene and a highly similar gene located nearby, which affect the highly conserved carbohydrate recognition domain, are associated with idiopathic pulmonary fibrosis. The current version of the assembly displays only a single centromeric SFTPA gene pair rather than the two gene pairs shown in the previous assembly which were thought to have resulted from a duplication. |
| Alternative Names | COLEC5 FLJ50594 FLJ50597 FLJ51953 FLJ79091 FLJ93678 MGC133169 MGC133366 MGC189714 MGC189761 PSAP SFTP1 SFTPA2B SPA2 SPAII |
| Molecular Weight(MW) | 26kDa |
| Sequence | 248 amino acids |
| Entrez Gene | 729238 |
|---|---|
| SwissProt ID | Q8IWL1 |
| Protein Accession | NP_001092138 |
Immunoblot
Lane 1: 25ng purified Human Alveolar sample (w/SP-A1+SP-A2) Lane 1: 25ng purified Human Alveolar sample (w/SP-A1+SP-A2)Lane 2: 20ug Rat bronchoalveolar lavageLane 3: 25ng hSP-A2 variant expressed in CHO cellsLane 4: 25ng hSP-A2 variant expressed in CHO cellsLane 5: 25ng hSP-A1/A2 variants expressed in CHO cellsLane 6: 25ng hSP-A1/A2 variants expressed in CHO cellsLane 7: 20ug SP-A1/2 KO mouse bronchoalveolar lavageLane 8: 20ug hSP-A2 variant transgenic mouse bronchoalveolar lavageLane 9: 20ug hSP-A2 variant transgenic mouse bronchoalveolar lavageLane 10: 20ug hSP-A1 variant transgenic mouse bronchoalveolar lavageLane 11: 20ug hSP-A1 variant transgenic mouse bronchoalveolar lavageLane 12: 20ug hSP-A1/A2 variants transgenic mouse bronchoalveolar lavageLane 13: 20ug mSP-A1/A2 bronchoalveolar lavage; +/- mouseLane 14: 20ug mSP-A1/A2 bronchoalveolar lavage; WT mouseLane 15: 10ug human alveolar cell lysateLane 16: 25ng purified hSP-A1/A2Primary Antibody Dilution: 1:2500Secondary Antibody: IgG HRP ConjSecondary Antibody Dilution: 1:10000Gene Name: SFTPA2Submitted by: Todd M. Umstead, Pennsylvania State University College of Medicine
Immunoblot
1: 20ng human SP-A2 protein, 2: 20ug rat wt BAL cell lysate, 3: 25ng hSP-A2 protein purified from transfected mouse CHO lysate, 4: 25ng hSP-A2 protein purified from transfected mouse CHO lysate, 5: 25ng hSP-A1 protein purified from transfected mouse CHO lysate, 6: 25ng hSP-A1 protein purified from transfected mouse CHO lysate, 7: 25ng hSP-A1/A2 protein purified from transfected mouse CHO lysate, 8: 25ng hSP-A1/A2 protein purified from transfected mouse CHO lysate, 9: 20ug SP-A KO mouse BAL lysate, 10: 25ng hSP-A2 protein purified from transfected mouse BAL lysate, 11: 25ng human SP-A2 protein purified from transfected mouse BAL lysate, 12: 25ng human SP-A1 protein purified from transfected mouse BAL lysate, 13: 25ng human SP-A1 protein purified from transfected mouse BAL lysate, 14: 25ng human SP-A1/2 protein purified from transfected mouse BAL lysate, 15: 25ng mouse SP-A2 protein purified from mouse BAL lysate, 16: 20ug mouse wt BAL lysate, 17: 15ug human wt T2 lysate, 18: 25ng human SP-A2 protein.Primary Antibody Dilution: 1:2500Secondary Antibody: goat anti-rabbit-HRPSecondary Antibody Dilution: 1:10,000Gene Name: SFTPA2Submitted by: Todd M. Umstead, David S. Phelps, Joanna Floros, Penn State Center for Host defense, Inflammation, and Lung Disease (CHILD) Research, Department of Pediatrics, The Pennsylvania State University College of Medicine
Western blot
Titration: 1.0 ug/mlPositive Control: Fetal Heart| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
