Immunofluorescence
Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from NIH-3T3 cells treated with UV 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The lane on the right is treated with the synthesized peptide| Product Name | 14-3-3 zeta (Phospho-Ser58) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta around the phosphorylation site of Ser58. |
| Modification | p-Ser58 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBIHCIFELISA |
| WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:40000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YWHAZ |
|---|---|
| Gene Synonyms | 14-3-3 protein zeta/delta 1433Z 143Z FAS Factor activating exoenzyme S KCIP-1 Mitochondrial import stimulation factor S1 subunit Protein kinase C inhibitor protein-1 YWHAZ |
| Entrez Gene | 7534 |
|---|---|
| Unigene | Hs.492407,Hs.594673 |
| OMIM | 601288 |
| SwissProt ID | P63104 |
Immunofluorescence
Immunofluorescence analysis of HeLa cells treated with PMA 125ng/ml 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 14-3-3 zeta (Phospho-Ser58) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from NIH-3T3 cells treated with UV 30', using 14-3-3 zeta (Phospho-Ser58) Antibody. The lane on the right is treated with the synthesized peptide| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:40000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
