Immunohistochemical
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Western blot analysis of extracts from MDA-MB-435 cells treated with EGF 200ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is treated with the synthesized peptide| Product Name | 4E-BP1 (Phospho-Thr45) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Thr45. |
| Modification | p-Thr45 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBIHCELISA |
| WB:1:500~1:1000 IHC:1:50~1:100 ELISA:1:20000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF4EBP1 |
|---|---|
| Gene Synonyms | 4EBP1 EIF4EBP1 Eukaryotic translation initiation factor 4E binding protein 1 Insulin-stimulated EIF-4E binding protein PHAS-I P/OKCL.6 PHAS-1 PHAS-I |
Immunohistochemical
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Western blot analysis of extracts from MDA-MB-435 cells treated with EGF 200ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is treated with the synthesized peptide| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 ELISA:1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
