Immunohistochemical
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Akt (Phospho-Ser473) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Western blot analysis of extracts from HeLa cells treated with heat shock, using Akt (Phospho-Ser473) Antibody. The lane on the left is treated with the synthesized peptide| Product Name | Rabbit anti-Akt (Phospho-Ser473) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser473. |
| Modification | p-Ser473 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBIHCELISA |
| WB:1:500~1:1000 IHC:1:50~1:100 ELISA:1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1 |
|---|---|
| Gene Synonyms | AKT AKT1 kinase C-AKT kinase Akt1 PKB PKB-alpha Protein kinase B RAC RAC-alpha serine/threonine kinase RAC-PK-alpha |
| Entrez Gene | 207 208 10000 |
|---|---|
| Unigene | 525622 631535 498292 |
| OMIM | 164730 164731 611223 |
| SwissProt ID | P31749 P31751 Q9Y243 |
Immunohistochemical
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Akt (Phospho-Ser473) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Western blot analysis of extracts from HeLa cells treated with heat shock, using Akt (Phospho-Ser473) Antibody. The lane on the left is treated with the synthesized peptide| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 ELISA:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
