Immunofluorescence
Immunofluorescence analysis of HuvEc cells, using BAX Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using BAX Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from 293 cells, using BAX Antibody. The lane on the right is treated with the synthesized peptideWB
(B) The expression of apoptosis-related proteins BAX, Bcl-2, and cytochrome C was detected by western blotting following different treatments (100 μmol/L berberine, 10 h incubation). Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). Analysis of variance and Student-Newman-Keuls post hoc tests were used to compare two group (vs. berberine group).| Product Name | Rabbit anti-BAX polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BAX. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCWB |
| WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:20000 |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAX |
|---|---|
| Gene Synonyms | Apoptosis regulator BAX membrane isoform alpha BAXA Bcl2-associated X protein |
Immunofluorescence
Immunofluorescence analysis of HuvEc cells, using BAX Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using BAX Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from 293 cells, using BAX Antibody. The lane on the right is treated with the synthesized peptide
WB
(B) The expression of apoptosis-related proteins BAX, Bcl-2, and cytochrome C was detected by western blotting following different treatments (100 μmol/L berberine, 10 h incubation). Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). Analysis of variance and Student-Newman-Keuls post hoc tests were used to compare two group (vs. berberine group).| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 168% glycerol. |
