Immunofluorescence
Immunofluorescence analysis of HeLa cells, using C-RAF (Phospho-Ser621) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C-RAF (Phospho-Ser621) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from HeLa cells treated with UV 5', using C-RAF (Phospho-Ser621) Antibody. The lane on the right is treated with the synthesized peptide| Product Name | Rabbit anti-C-RAF (Phospho-Ser621) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser621. |
| Modification | p-Ser621 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBIHCIFELISA |
| WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAF1 |
|---|---|
| Gene Synonyms | C-RAF C-Raf CRAF kinase Raf1 Protein kinase raf 1 RAF proto-oncogene serine/threonine-protein kinase Raf proto-oncogene serine/threonine-protein kinase RAF-1 Raf-1 RAF1 Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 |
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using C-RAF (Phospho-Ser621) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C-RAF (Phospho-Ser621) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from HeLa cells treated with UV 5', using C-RAF (Phospho-Ser621) Antibody. The lane on the right is treated with the synthesized peptide| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
