Western blot
Western blot analysis of extracts from HuvEc cells treated with TNF 20ng/ml 30', using HSP27 (Phospho-Ser82) Antibody. The lane on the right is treated with the synthesized peptide| Product Name | Rabbit anti-HSP27 (Phospho-Ser82) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP27 around the phosphorylation site of Ser82. |
| Modification | p-Ser82 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBELISA |
| WB:1:500~1:1000 ELISA:1:4000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HSPB1 |
|---|---|
| Gene Synonyms | 28 kDa heat shock protein Estrogen-regulated 24 kDa protein Growth-related 25 kDa protein Heat shock 27 kDa protein HSP 27 HSP25 HSPB1 P25 SRP27 Stress-responsive protein 27 |
Western blot
Western blot analysis of extracts from HuvEc cells treated with TNF 20ng/ml 30', using HSP27 (Phospho-Ser82) Antibody. The lane on the right is treated with the synthesized peptide| Application Notes | WB:1:500~1:1000 ELISA:1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
