Immunohistochemical
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr254) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Western blot analysis of extracts from 293 cells treated with TNF-alpha, using NF-kappaB p65 (Phospho-Thr254) Antibody. The lane on the left is treated with the synthesized peptide| Product Name | Rabbit anti-NF-kappaB p65 (Phospho-Thr254) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Thr254. |
| Modification | p-Thr254 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBIHCIPELISA |
| WB:1:500~1:1000 IHC:1:50~1:100 ELISA:1:20000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RELA |
|---|---|
| Gene Synonyms | NFKB3 nuclear factor NF-kappa-B p65 subunit p65 RELA TF65 transcription factor p65 |
Immunohistochemical
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 (Phospho-Thr254) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Western blot analysis of extracts from 293 cells treated with TNF-alpha, using NF-kappaB p65 (Phospho-Thr254) Antibody. The lane on the left is treated with the synthesized peptide| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 ELISA:1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
