Immunofluorescence
Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NPM (Phospho-Thr199) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NPM (Phospho-Thr199) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from HepG2 cells, using NPM (Phospho-Thr199) Antibody. The lane on the right is treated with the synthesized peptide| Product Name | Rabbit anti-NPM (Phospho-Thr199) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. |
| Modification | p-Thr199 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBIHCIFELISA |
| WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:60000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NPM1 |
|---|---|
| Gene Synonyms | B23.1 NPM isoform 1 NPM1 Nucleolar phosphoprotein B23 Nucleolar protein NO38 nucleophosmin nucleophosmin (nucleolar phosphoprotein B23 numatrin) Numatrin |
Immunofluorescence
Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using NPM (Phospho-Thr199) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NPM (Phospho-Thr199) Antibody. The picture on the right is treated with the synthesized peptide.Western blot analysis of extracts from HepG2 cells, using NPM (Phospho-Thr199) Antibody. The lane on the right is treated with the synthesized peptide| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 IF:1:100~1:500 ELISA:1:60000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
