Immunofluorescence
Immunofluorescence analysis of NIH-3T3 cells, using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Western blot analysis of extracts from COS7 cells treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is treated with the synthesized peptideWB
e Representative western blot images and quantitative analysis of TGF-β, PI3K-P85, p-AKT, AKT and mTOR.| Product Name | Rabbit anti-PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PI3-kinase p85-alpha/gamma around the phosphorylation site of Tyr467/199. |
| Modification | p-Tyr467/199 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| WB:1:500~1:1000 IF:1:100~1:500 ELISA:1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIK3R1 |
|---|---|
| Gene Synonyms | GRB1 P85A PI3-kinase p85-alpha subunit PI3K PI3K p85-alpha Phosphatidylinositol 3-kinase regulatory alpha subunit PtdIns-3-kinase p85-alpha P55G PI3-kinase p85-gamma subunit PI3K p85-gamma Phosphatidylinositol 3-kinase regulatory gamma subunit P |
Immunofluorescence
Immunofluorescence analysis of NIH-3T3 cells, using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Western blot analysis of extracts from COS7 cells treated with H2O2 100uM 30', using PI3-kinase p85-alpha/gamma (Phospho-Tyr467/199) Antibody. The lane on the right is treated with the synthesized peptide
WB
e Representative western blot images and quantitative analysis of TGF-β, PI3K-P85, p-AKT, AKT and mTOR.| Application Notes | WB:1:500~1:1000 IF:1:100~1:500 ELISA:1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
