Immunofluorescence
Immunofluorescence analysis of A549 cells, using S1P Receptor EDG1 (Ab-236) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Western blot analysis of extracts from HT-29/LOVO/A549 cells, using S1P Receptor EDG1 (Ab-236) Antibody. The lane on the right is treated with the synthesized peptideWB
C, Western blot images and their quantification of sphingosine-1-phosphate receptor-3 (S1PR1-3) accumulation in the colon muscle layers from different groups of rats.IF
B, Representative double immunofluorescence staining of colon smooth muscle (CSM) for α-actin and sphingosine-1-phosphate receptor-3 (S1PR1-3). Nuclei were identified by DAPI staining.| Product Name | Rabbit anti-S1P Receptor EDG1 polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Immunogen | The antiserum was produced against synthesized peptide derived from human S1P Receptor EDG1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| WB:1:500~1:1000 IF:1:100~1:500 ELISA:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | S1PR1 |
|---|---|
| Gene Synonyms | Sphingosine 1-phosphate receptor Edg-1 Sphingosine 1-phosphate receptor 1 S1P1 EDG1 |
Immunofluorescence
Immunofluorescence analysis of A549 cells, using S1P Receptor EDG1 (Ab-236) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Western blot analysis of extracts from HT-29/LOVO/A549 cells, using S1P Receptor EDG1 (Ab-236) Antibody. The lane on the right is treated with the synthesized peptide
WB
C, Western blot images and their quantification of sphingosine-1-phosphate receptor-3 (S1PR1-3) accumulation in the colon muscle layers from different groups of rats.
IF
B, Representative double immunofluorescence staining of colon smooth muscle (CSM) for α-actin and sphingosine-1-phosphate receptor-3 (S1PR1-3). Nuclei were identified by DAPI staining.| Application Notes | WB:1:500~1:1000 IF:1:100~1:500 ELISA:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
