Immunohistochemical
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad3 (Phospho-Ser425) Antibody. The picture on the right is treated with the synthesized peptide.Western blot
Western blot analysis of extracts from NIH-3T3 cells treated with IGF-I 100ng/ml 10', using Smad3 (Phospho-Ser425) Antibody. The lane on the right is treated with the synthesized peptide| Product Name | Rabbit anti-Smad3 (Phospho-Ser425) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser425. |
| Modification | p-Ser425 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WBIHCELISA |
| WB:1:500~1:1000 IHC:1:50~1:100 ELISA:1:40000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SMAD3 |
|---|---|
| Gene Synonyms | JV15-2 MAD-3 MADH3 Mad3 Mothers against DPP homolog 3 Mothers against decapentaplegic homolog 3 SMAD 3 Smad 3 |
Immunohistochemical
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Smad3 (Phospho-Ser425) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot
Western blot analysis of extracts from NIH-3T3 cells treated with IGF-I 100ng/ml 10', using Smad3 (Phospho-Ser425) Antibody. The lane on the right is treated with the synthesized peptide| Application Notes | WB:1:500~1:1000 IHC:1:50~1:100 ELISA:1:40000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
