Application
LRWD1 Antibody (N-term) (Cat. #AP11807a) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LRWD1 antibody detected the LRWD1 protein (arrow).Application
LRWD1 Antibody (N-term) (Cat. #AP11807a)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LRWD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | LRWD1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LRWD1; ORCA; Leucine-rich repeat and WD repeat-containing protein 1; ORC-associated protein; Origin recognition complex-associated protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ORCA |
|---|---|
| Alternative Names | LRWD1 ORCA Leucine-rich repeat and WD repeat-containing protein 1 ORC-associated protein Origin recognition complex-associated protein |
| Molecular Weight(MW) | 70861 Da |
| Function | Associated component of the origin recognition complex (ORC) required to recruit and stabilize the ORC complex to chromatin. Probably required for the association of ORC on chromatin during G1 to establish pre-replication complex (preRC) and to heterochromatic sites in post-replicated cells. Binds a combination of DNA and histone methylation repressive marks on heterochromatin: binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 in a cooperative manner with DNA methylation. It is unclear whether it recognizes and binds these repressive marks by itself or needs additional factors |
| Tissue Specificity | This LRWD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-33 amino acids from the N-terminal region of human LRWD1. |
| Cellular Localization | Nucleus. Chromosome, centromere. Chromosome, telomere. Note=Localizes to heterochromatin during G1 phase Restricted to centromeres or telomeres as cells progress though S phase. When cells enter mitosis, relocalizes to centromeres |
| Entrez Gene | 222229 |
|---|---|
| Nucleotide Accession | NP_690852.1 |
Application
LRWD1 Antibody (N-term) (Cat. #AP11807a) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LRWD1 antibody detected the LRWD1 protein (arrow).
Application
LRWD1 Antibody (N-term) (Cat. #AP11807a)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LRWD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
