Application
Western blot analysis of Adenosine A3-R expression in non-transfected 293T: (A), human Adenosine A3-R transfected 293T: (B) and SH-SY5Y (C) whole cell lysates.Application
Western blot analysis of Adenosine A3-R expression in non-transfected 293T: (A), human Adenosine A3-R transfected 293T: (B) and T98G (C) whole cell lysates.Application
Western blot analysis of Adenosine A3-R expression in non-transfected 293T: (A), human Adenosine A3-R synthase transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.Application
Western blot analysis of Adenosine A3-R expression in T98G whole cell lysate.| Product Name | Adenosine A3-R Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A3AR,A3R,AA3R,AD026,Adenosine A3 receptor,ADORA 3,ADORA3,ARA3 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Adenosine is involved in a variety of processes, including the synthesis of urea, the anti-inflammatory response, and the inhibition of protein synthesis. The Adenosine receptors, including Adenosine A1-R, Adenosine A2A-R, Adenosine A2B-R and Adenosine A3-R, are integral membrane proteins that are members of the G protein-coupled receptor family. Adenosine A1-R mediates ureagenesis in a partially calcium-dependent manner. Adenosine is known to mediate coronary vasodilation via Adenosine A2A-R. Collagen synthesis and total protein synthesis are inhibited in certain cells by Adenosine, acting via the A2B receptors. Activation of Adenosine A3-R inhibits the induction of TNFå and blocks the endotoxin CD14 receptor signal transduction pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | canine,porcine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADORA3 |
|---|---|
| Gene Synonyms | AD026 |
| Alternative Names | A3AR A3R AA3R AD026 Adenosine A3 receptor ADORA 3 ADORA3 ARA3 |
| Molecular Weight(MW) | 35 kDa |
| Tissue Specificity | epitope corresponding to amino acids 151-230 mapping within an internal region of Adenosine A3 receptor of human origin |
| Entrez Gene | 140 |
|---|
Application
Western blot analysis of Adenosine A3-R expression in non-transfected 293T: (A), human Adenosine A3-R transfected 293T: (B) and SH-SY5Y (C) whole cell lysates.
Application
Western blot analysis of Adenosine A3-R expression in non-transfected 293T: (A), human Adenosine A3-R transfected 293T: (B) and T98G (C) whole cell lysates.
Application
Western blot analysis of Adenosine A3-R expression in non-transfected 293T: (A), human Adenosine A3-R synthase transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.
Application
Western blot analysis of Adenosine A3-R expression in T98G whole cell lysate.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
