Application
Western blot analysis of MAP2K1 (arrow) using rabbit polyclonal MAP2K1-pT285.ctrl . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K1 gene (Lane 2) .Application
MAP2K1 Antibody (T285) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAP2K1 Antibody (T285) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MAP2K1 Antibody (T285) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MAP2K1 Antibody (T285) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MEK1 PRKMK1 |
|---|---|
| Alternative Names | MAP2K1 MEK1 PRKMK1 Dual specificity mitogen-activated protein kinase kinase 1 ERK activator kinase 1 MAPK/ERK kinase 1 |
| Molecular Weight(MW) | 43439 Da |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator- activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis |
| Tissue Specificity | Widely expressed, with extremely low levels in brain |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole body. Cytoplasm. Nucleus Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis |
| Entrez Gene | 5604 |
|---|
Application
Western blot analysis of MAP2K1 (arrow) using rabbit polyclonal MAP2K1-pT285.ctrl . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K1 gene (Lane 2) .
Application
MAP2K1 Antibody (T285) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAP2K1 Antibody (T285) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MAP2K1 Antibody (T285) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
