Application
Western blot analysis of MAPK3 Antibody (N-term) Pab in HepG2 cell line lysates (35ug/lane). MAPK3(arrow) was detected using the purified polyclonal antibody.Application
Western blot analysis of MAPK3 (arrow) using purified Pab . 293 cell lysates (1 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK3 gene (Lane 2) (Origene Technologies).Application
MAPK3 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MAPK3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; ERT2; Extracellular signal-regulated kinase 1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; Microtubule-associated protein 2 kinase; p44-ERK1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ERK1 PRKM3 |
|---|---|
| Alternative Names | MAPK3 ERK1 PRKM3 Mitogen-activated protein kinase 3 ERT2 Extracellular signal-regulated kinase 1 Insulin-stimulated MAP2 kinase MAP kinase isoform p44 Microtubule-associated protein 2 kinase p44-ERK1 |
| Molecular Weight(MW) | 43136 Da |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10) Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade |
| Tissue Specificity | This ERK1 (MAPK3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51~81 amino acids from the N-terminal region of human MAPK3. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Autophosphorylation at Thr-207 promotes nuclear localization |
| Entrez Gene | 5595 |
|---|
Application
Western blot analysis of MAPK3 Antibody (N-term) Pab in HepG2 cell line lysates (35ug/lane). MAPK3(arrow) was detected using the purified polyclonal antibody.
Application
Western blot analysis of MAPK3 (arrow) using purified Pab . 293 cell lysates (1 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK3 gene (Lane 2) (Origene Technologies).
Application
MAPK3 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
