Application
Western blot analysis of anti-ERK5 Antibody (T218/Y220) in Ramos cell line lysates (35ug/lane). ERK5 (arrow) was detected using the purified Pab.Application
ERK5 Antibody (T218/Y220) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERK5 Antibody (T218/Y220) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | MAPK7 Antibody (T218/Y220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAPK7; BMK1; ERK5; PRKM7; Mitogen-activated protein kinase 7; Big MAP kinase 1; Extracellular signal-regulated kinase 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BMK1 ERK5 PRKM7 |
|---|---|
| Alternative Names | MAPK7 BMK1 ERK5 PRKM7 Mitogen-activated protein kinase 7 Big MAP kinase 1 Extracellular signal-regulated kinase 5 |
| Molecular Weight(MW) | 88386 Da |
| Function | Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras- independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression |
| Tissue Specificity | Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates to the nucleus upon activation |
| Entrez Gene | 5598 |
|---|
Application
Western blot analysis of anti-ERK5 Antibody (T218/Y220) in Ramos cell line lysates (35ug/lane). ERK5 (arrow) was detected using the purified Pab.
Application
ERK5 Antibody (T218/Y220) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ERK5 Antibody (T218/Y220) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
