Application
Western blot analysis of MAPKAP1 Antibody (C-term) in WiDr cell line lysates (35ug/lane). MAPKAP1 (arrow) was detected using the purified Pab.Application
Western blot analysis of MAPKAP1 (arrow) using rabbit polyclonal MAPKAP1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPKAP1 gene (Lane 2) .| Product Name | MAPKAP1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAPKAP1; MIP1; SIN1; Target of rapamycin complex 2 subunit MAPKAP1; Mitogen-activated protein kinase 2-associated protein 1; Stress-activated map kinase-interacting protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MIP1 SIN1 |
|---|---|
| Alternative Names | MAPKAP1 MIP1 SIN1 Target of rapamycin complex 2 subunit MAPKAP1 Mitogen-activated protein kinase 2-associated protein 1 Stress-activated map kinase-interacting protein 1 |
| Molecular Weight(MW) | 59123 Da |
| Function | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription |
| Tissue Specificity | Ubiquitously expressed, with highest levels in heart and skeletal muscle |
| Cellular Localization | Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus |
| Entrez Gene | 79109 |
|---|
Application
Western blot analysis of MAPKAP1 Antibody (C-term) in WiDr cell line lysates (35ug/lane). MAPKAP1 (arrow) was detected using the purified Pab.
Application
Western blot analysis of MAPKAP1 (arrow) using rabbit polyclonal MAPKAP1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPKAP1 gene (Lane 2) .| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
