Application
Western blot analysis of MCA1 (arrow) using rabbit polyclonal MCA1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MCA1 gene (Lane 2) (Origene Technologies).Application
MCA1 Antibody (N-term) (RB18766) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MCA1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MCA1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIMP1; EMAP2; SCYE1; Aminoacyl tRNA synthase complex-interacting multifunctional protein 1; Multisynthase complex auxiliary component p43; Endothelial monocyte-activating polypeptide 2; Endothelial monocyte-activating polypeptide II; Small inducible cytoki |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | EMAP2 SCYE1 |
|---|---|
| Alternative Names | AIMP1 EMAP2 SCYE1 Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 Multisynthase complex auxiliary component p43 Endothelial monocyte-activating polypeptide 2 Endothelial monocyte-activating polypeptide II Small inducible cytoki |
| Molecular Weight(MW) | 34353 Da |
| Function | Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1- mediated retention of HSP90B1/gp96 in the endoplasmic reticulum Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7 |
| Tissue Specificity | This MCA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~31 amino acids from the N-terminal region of human MCA1. |
| Cellular Localization | Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle, secretory vesicle (By similarity). Secreted (By similarity). Endoplasmic reticulum (By similarity). Golgi apparatus (By similarity). Note=Enriched in secretory vesicles of pancreatic alpha cells and secreted from the pancreas in response to low glucose levels (By similarity). Also secreted in response to hypoxia and both apoptotic and necrotic cell death |
| Entrez Gene | 9255 |
|---|
Application
Western blot analysis of MCA1 (arrow) using rabbit polyclonal MCA1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MCA1 gene (Lane 2) (Origene Technologies).
Application
MCA1 Antibody (N-term) (RB18766) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MCA1 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
