Application
Western blot analysis of MCM2 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). MCM2 (arrow) was detected using the purified Pab.Application
MCM2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCM2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | MCM2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BM28 CCNL1 CDCL1 KIAA0030 |
|---|---|
| Alternative Names | MCM2 BM28 CCNL1 CDCL1 KIAA0030 DNA replication licensing factor MCM2 Minichromosome maintenance protein 2 homolog Nuclear protein BM28 |
| Molecular Weight(MW) | 101896 Da |
| Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division |
| Tissue Specificity | This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656~685 amino acids from the C-terminal region of human MCM2. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4171 |
|---|
Application
Western blot analysis of MCM2 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). MCM2 (arrow) was detected using the purified Pab.
Application
MCM2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MCM2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
