Application
Western blot analysis of MCM2 Antibody (Center) in 293 cell line lysates (35ug/lane). MCM2 (arrow) was detected using the purified Pab.Application
MCM2 Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MCM2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BM28 CCNL1 CDCL1 KIAA0030 |
|---|---|
| Alternative Names | MCM2 BM28 CCNL1 CDCL1 KIAA0030 DNA replication licensing factor MCM2 Minichromosome maintenance protein 2 homolog Nuclear protein BM28 |
| Molecular Weight(MW) | 101896 Da |
| Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division |
| Tissue Specificity | This MCM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495~524 amino acids from the Center region of human MCM2. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4171 |
|---|
Application
Western blot analysis of MCM2 Antibody (Center) in 293 cell line lysates (35ug/lane). MCM2 (arrow) was detected using the purified Pab.
Application
MCM2 Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
