Application
MCM4 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MCM4 antibody detected the MCM4 protein (arrow).Application
MCM4 Antibody (C-term) (AP14534b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCM4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | MCM4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCM4; CDC21; DNA replication licensing factor MCM4; CDC21 homolog; P1-CDC21 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDC21 |
|---|---|
| Alternative Names | MCM4 CDC21 DNA replication licensing factor MCM4 CDC21 homolog P1-CDC21 |
| Molecular Weight(MW) | 96558 Da |
| Function | Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity |
| Tissue Specificity | This MCM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636-665 amino acids from the C-terminal region of human MCM4. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 4173 |
|---|---|
| Nucleotide Accession | NP_005905.2 NP_877423.1 |
Application
MCM4 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MCM4 antibody detected the MCM4 protein (arrow).
Application
MCM4 Antibody (C-term) (AP14534b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCM4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
