Application
Western blot analysis of anti-MEK5 Pab in Ramos cell lysate. MEK5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human heart muscle tissue reacted with MEK5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | MEK5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP2K5; MEK5; MKK5; PRKMK5; Dual specificity mitogen-activated protein kinase kinase 5; MAPK/ERK kinase 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MEK5 MKK5 PRKMK5 |
|---|---|
| Alternative Names | MAP2K5 MEK5 MKK5 PRKMK5 Dual specificity mitogen-activated protein kinase kinase 5 MAPK/ERK kinase 5 |
| Molecular Weight(MW) | 50112 Da |
| Function | Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis |
| Tissue Specificity | Expressed in many adult tissues. Abundant in heart and skeletal muscle |
| Entrez Gene | 5607 |
|---|
Application
Western blot analysis of anti-MEK5 Pab in Ramos cell lysate. MEK5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human heart muscle tissue reacted with MEK5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
