Application
Western blot analysis of anti-MEKK3 Antibody (N-term) in T47D cell line lysates (35ug/lane). MEKK3 (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MEKK3 Antibody (N-term E29), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | MEKK3 Antibody (N-term E29) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP3K3; MAPKKK3; MEKK3; Mitogen-activated protein kinase kinase kinase 3; MAPK/ERK kinase kinase 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAPKKK3 MEKK3 |
|---|---|
| Alternative Names | MAP3K3 MAPKKK3 MEKK3 Mitogen-activated protein kinase kinase kinase 3 MAPK/ERK kinase kinase 3 |
| Molecular Weight(MW) | 70898 Da |
| Function | Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators |
| Tissue Specificity | This MEKK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22~51 amino acids from the N-terminal(E29) region of human MEKK3. |
| Entrez Gene | 4215 |
|---|
Application
Western blot analysis of anti-MEKK3 Antibody (N-term) in T47D cell line lysates (35ug/lane). MEKK3 (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MEKK3 Antibody (N-term E29), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
