Application
The Mib Pab (Cat.# AP2172a) is used at 1:300 to detect FLAG-tagged Mib in 293T cell lysate. Data courtesy of Dr. Olga Ossipova, Mt. Sinai School of Medicine.Application
Immunofluorescent staining of Hela cells incubated with MIB Antibody(N-term) (Cat # AP2172a) at a dilution of 1:20. Data courtesy of Dr.Vyacheslav Akimov, University of Southern Denmark.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody,which was peroxidase-conjugated to the secondary antibody, followed by AECstaining. This data demonstrates the use of this antibody forimmunohistochemistry; clinical relevance has not been evaluated. BC = breastcarcinoma; HC = hepatocarcinoma.| Product Name | Mib1/Mindbomb Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MIB1; DIP1; KIAA1323; ZZANK2; E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; Mind bomb homolog 1; Zinc finger ZZ type with ankyrin repeat domain protein 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | DIP1 KIAA1323 ZZANK2 |
|---|---|
| Alternative Names | MIB1 DIP1 KIAA1323 ZZANK2 E3 ubiquitin-protein ligase MIB1 DAPK-interacting protein 1 Mind bomb homolog 1 Zinc finger ZZ type with ankyrin repeat domain protein 2 |
| Molecular Weight(MW) | 110136 Da |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis (By similarity). Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation |
| Tissue Specificity | Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined |
| Cellular Localization | Cytoplasm. Cell membrane (By similarity). Note=Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear |
| Entrez Gene | 57534 |
|---|
Application
The Mib Pab (Cat.# AP2172a) is used at 1:300 to detect FLAG-tagged Mib in 293T cell lysate. Data courtesy of Dr. Olga Ossipova, Mt. Sinai School of Medicine.
Application
Immunofluorescent staining of Hela cells incubated with MIB Antibody(N-term) (Cat # AP2172a) at a dilution of 1:20. Data courtesy of Dr.Vyacheslav Akimov, University of Southern Denmark.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody,which was peroxidase-conjugated to the secondary antibody, followed by AECstaining. This data demonstrates the use of this antibody forimmunohistochemistry; clinical relevance has not been evaluated. BC = breastcarcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
