Application
Western blot analysis of anti-MINA (C-term) Pab pre-incubated with and without blocking peptide (BP)(catlog #:BP1033b) in Jurkat cell line lysate. MINA(C-term)(arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MINA (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of MINA (arrow) using rabbit polyclonal MINA Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MINA gene.| Product Name | MINA Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MINA; MDIG; MINA53; NO52; MYC-induced nuclear antigen; Mineral dust-induced gene protein; Nucleolar protein 52 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MDIG MINA53 NO52 |
|---|---|
| Alternative Names | MINA MDIG MINA53 NO52 MYC-induced nuclear antigen Mineral dust-induced gene protein Nucleolar protein 52 |
| Molecular Weight(MW) | 52800 Da |
| Function | Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Is involved in the demethylation of trimethylated 'Lys-9' on histone H3 (H3K9me3), leading to an increase in ribosomal RNA expression Also catalyzes the hydroxylation of 60S ribosomal protein L27a on 'His-39'. May play an important role in cell growth and survival May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles |
| Tissue Specificity | Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines |
| Cellular Localization | Nucleus. Nucleus, nucleolus. |
| Entrez Gene | 84864 |
|---|
Application
Western blot analysis of anti-MINA (C-term) Pab pre-incubated with and without blocking peptide (BP)(catlog #:BP1033b) in Jurkat cell line lysate. MINA(C-term)(arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MINA (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of MINA (arrow) using rabbit polyclonal MINA Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MINA gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
