Application
MKK7-S271/T275 western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the JW antibody detected the JW protein (arrow).Application
MKK7-S271/T275 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MKK7-S271/T275 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
MKK7-S271/T275 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MKK7-S271/T275 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAP2K7; JNKK2; MEK7; MKK7; PRKMK7; SKK4; Dual specificity mitogen-activated protein kinase kinase 7; JNK-activating kinase 2; MAPK/ERK kinase 7; Stress-activated protein kinase kinase 4; c-Jun N-terminal kinase kinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | JNKK2 MEK7 MKK7 PRKMK7 SKK4 |
|---|---|
| Alternative Names | MAP2K7 JNKK2 MEK7 MKK7 PRKMK7 SKK4 Dual specificity mitogen-activated protein kinase kinase 7 JNK-activating kinase 2 MAPK/ERK kinase 7 Stress-activated protein kinase kinase 4 c-Jun N-terminal kinase kinase 2 |
| Molecular Weight(MW) | 47485 Da |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis |
| Tissue Specificity | Ubiquitous; with highest level of expression in skeletal muscle. Isoform 3 is found at low levels in placenta, fetal liver, and skeletal muscle |
| Cellular Localization | Nucleus. Cytoplasm (By similarity). |
| Entrez Gene | 5609 |
|---|---|
| Nucleotide Accession | NP_660186.1 |
Application
MKK7-S271/T275 western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the JW antibody detected the JW protein (arrow).
Application
MKK7-S271/T275 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MKK7-S271/T275 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MKK7-S271/T275 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
