Application
Fluorescent confocal image of SY5Y cells stained with MSX1 (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP19261c MSX1 (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the MSX1 mainly to the nucleus.Application
MSX1 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).Application
MSX1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).| Product Name | MSX1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MSX1; HOX7; Homeobox protein MSX-1; Homeobox protein Hox-7; Msh homeobox 1-like protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| IF:1:200 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HOX7 |
|---|---|
| Alternative Names | MSX1 HOX7 Homeobox protein MSX-1 Homeobox protein Hox-7 Msh homeobox 1-like protein |
| Molecular Weight(MW) | 30940 Da |
| Function | Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity |
| Tissue Specificity | Expressed in the developing nail bed mesenchyme |
| Cellular Localization | Nucleus. |
| Entrez Gene | 4487 |
|---|---|
| Nucleotide Accession | NP_002439.2 |
Application
Fluorescent confocal image of SY5Y cells stained with MSX1 (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP19261c MSX1 (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the MSX1 mainly to the nucleus.
Application
MSX1 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).
Application
MSX1 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the MSX1 antibody detected the MSX1 protein (arrow).| Application Notes | IF:1:200 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
