Application
Western blot analysis of MTHFD2 antibody (C-term) (Cat.# AP7362b) in NCI-H460 cell line lysates (35ug/lane). MTHFD2 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human Colon carcinoma reacted with MTHFD2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
MTHFD2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MTHFD2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MTHFD2; NMDMC; Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; NAD-dependent methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NMDMC |
|---|---|
| Alternative Names | MTHFD2 NMDMC Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase mitochondrial NAD-dependent methylenetetrahydrofolate dehydrogenase Methenyltetrahydrofolate cyclohydrolase |
| Molecular Weight(MW) | 37895 Da |
| Tissue Specificity | This MTHFD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278~307 amino acids from the C-terminal region of human MTHFD2. |
| Cellular Localization | Mitochondrion. |
| Entrez Gene | 10797 |
|---|
Application
Western blot analysis of MTHFD2 antibody (C-term) (Cat.# AP7362b) in NCI-H460 cell line lysates (35ug/lane). MTHFD2 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human Colon carcinoma reacted with MTHFD2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
MTHFD2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
