Application
NANOS1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NANOS1 antibody detected the NANOS1 protein (arrow).Application
NANOS1 Antibody (Center) (AP14401c)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOS1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | NANOS1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NANOS1; NOS1; Nanos homolog 1; EC_Rep1a |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NOS1 |
|---|---|
| Alternative Names | NANOS1 NOS1 Nanos homolog 1 EC_Rep1a |
| Molecular Weight(MW) | 30230 Da |
| Function | May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin Up-regulates the production of MMP14 to promote tumor cell invasion |
| Tissue Specificity | Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness |
| Cellular Localization | Cytoplasm, perinuclear region. Cytoplasm. Note=Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells |
| Entrez Gene | 340719 |
|---|---|
| Nucleotide Accession | NP_955631.1 |
Application
NANOS1 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NANOS1 antibody detected the NANOS1 protein (arrow).
Application
NANOS1 Antibody (Center) (AP14401c)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NANOS1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
