Application
Western blot analysis of NAT1 Antibody (C-term) in CEM cell line lysates (35ug/lane).NAT1 (arrow) was detected using the purified Pab.Application
NAT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NAT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
NAT1 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of NAT1 Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | NAT1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NAT1; AAC1; Arylamine N-acetyltransferase 1; Arylamide acetylase 1; Monomorphic arylamine N-acetyltransferase; N-acetyltransferase type 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AAC1 |
|---|---|
| Alternative Names | NAT1 AAC1 Arylamine N-acetyltransferase 1 Arylamide acetylase 1 Monomorphic arylamine N-acetyltransferase N-acetyltransferase type 1 |
| Molecular Weight(MW) | 33899 Da |
| Function | Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens |
| Tissue Specificity | This NAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-296 amino acids from the C-terminal region of human NAT1. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 9 |
|---|---|
| Nucleotide Accession | NP_000653.3 |
Application
Western blot analysis of NAT1 Antibody (C-term) in CEM cell line lysates (35ug/lane).NAT1 (arrow) was detected using the purified Pab.
Application
NAT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NAT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
NAT1 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of NAT1 Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
