Application
Fluorescent confocal image of HeLa cells stained with Natriuretic Peptide Receptor C (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8113a Natriuretic Peptide Receptor C (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the Natriuretic Peptide Receptor C mainly to the mainly to the nucleus.Application
Western blot analysis of ANPC-S82 in NCI-H460,HL-60 cell line lysates (35ug/lane). ANPC (arrow) was detected using the purified Pab.Application
Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Natriuretic Peptide Receptor C Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NPR3; ANPRC; C5orf23; NPRC; Atrial natriuretic peptide receptor 3; Atrial natriuretic peptide clearance receptor; Atrial natriuretic peptide receptor type C |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBIHC |
| IF:1:200 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ANPRC C5orf23 NPRC |
|---|---|
| Alternative Names | NPR3 ANPRC C5orf23 NPRC Atrial natriuretic peptide receptor 3 Atrial natriuretic peptide clearance receptor Atrial natriuretic peptide receptor type C |
| Molecular Weight(MW) | 59808 Da |
| Function | Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity |
| Tissue Specificity | This Natriuretic Peptide Receptor C Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75~104 amino acids from the N-terminal region of human ANPC. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 4883 |
|---|
Application
Fluorescent confocal image of HeLa cells stained with Natriuretic Peptide Receptor C (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8113a Natriuretic Peptide Receptor C (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the Natriuretic Peptide Receptor C mainly to the mainly to the nucleus.
Application
Western blot analysis of ANPC-S82 in NCI-H460,HL-60 cell line lysates (35ug/lane). ANPC (arrow) was detected using the purified Pab.
Application
Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | IF:1:200 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
