Application
Western blot analysis of NCF4 antibody (C-term) in K562 cell line lysates (35ug/lane). NCF4 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
NCF4 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NCF4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCF4; SH3PXD4; Neutrophil cytosol factor 4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40-phox |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SH3PXD4 |
|---|---|
| Alternative Names | NCF4 SH3PXD4 Neutrophil cytosol factor 4 Neutrophil NADPH oxidase factor 4 SH3 and PX domain-containing protein 4 p40-phox |
| Molecular Weight(MW) | 39032 Da |
| Function | Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex |
| Tissue Specificity | Expression is restricted to hematopoietic cells |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 4689 |
|---|
Application
Western blot analysis of NCF4 antibody (C-term) in K562 cell line lysates (35ug/lane). NCF4 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
NCF4 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
