Application
The anti-NEK9 Pab is used in Western blot to detect NEK9 in 293 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | NEK9 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NEK9; KIAA1995; NEK8; NERCC; Serine/threonine-protein kinase Nek9; Nercc1 kinase; Never in mitosis A-related kinase 9; NimA-related kinase 8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1995 NEK8 NERCC |
|---|---|
| Alternative Names | NEK9 KIAA1995 NEK8 NERCC Serine/threonine-protein kinase Nek9 Nercc1 kinase Never in mitosis A-related kinase 9 NimA-related kinase 8 |
| Molecular Weight(MW) | 107168 Da |
| Function | Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues Important for G1/S transition and S phase progression Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively |
| Tissue Specificity | Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 91754 |
|---|
Application
The anti-NEK9 Pab is used in Western blot to detect NEK9 in 293 cell lysate (Lane 1) and mouse heart tissue lysate (Lane 2).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
