Application
A. Western blot analysis on SHSY5Y cell lysate using anti-NF-66 RabMAb (Cat. #AJ1533a).Application
B. Immunofluorescent staining of PC 12 cells using anti-NF-66 RabMAb (Cat. #AJ1533a)Application
C. Positive immunohistochemical staining of paraffin-embedded human brain tissue (A.) using anti-NF-66 RabMAb.Application
D. Negative immunohistochemical staining of paraffin-embedded (D) human colon (E), Kidney (F) and uterus (D) tissues using NF-66 RabMAb (Cat#. 1674-1). Tissues were stained in parallel on the same Normal Tissue ArrayApplication
E. KidneyApplication
F. Uterus| Product Name | Neurofilament-66 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | INA, NEF5, Alpha-internexin, 66 kDa neurofilament protein;Neurofilament-66 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP676Y |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:1000~5000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NEF5 |
|---|---|
| Alternative Names | INA NEF5 Alpha-internexin 66 kDa neurofilament protein Neurofilament-66 |
| Molecular Weight(MW) | 55391 Da |
| Function | Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges |
| Tissue Specificity | Found predominantly in adult CNS. |
| Entrez Gene | 9118 |
|---|
Application
A. Western blot analysis on SHSY5Y cell lysate using anti-NF-66 RabMAb (Cat. #AJ1533a).
Application
B. Immunofluorescent staining of PC 12 cells using anti-NF-66 RabMAb (Cat. #AJ1533a)
Application
C. Positive immunohistochemical staining of paraffin-embedded human brain tissue (A.) using anti-NF-66 RabMAb.
Application
D. Negative immunohistochemical staining of paraffin-embedded (D) human colon (E), Kidney (F) and uterus (D) tissues using NF-66 RabMAb (Cat#. 1674-1). Tissues were stained in parallel on the same Normal Tissue Array
Application
E. Kidney
Application
F. Uterus| Application Notes | WB:1:1000~5000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
