Application
NFATC4 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NFATC4 antibody detected the NFATC4 protein (arrow).Application
Fluorescent confocal image of U251 cell stained with NFATC4 Antibody (C-term)(Cat#AP13162b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFATC4 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFATC4 immunoreactivity is localized to Cytoplasm significantly.| Product Name | NFATC4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFATC4; NFAT3; Nuclear factor of activated T-cells, cytoplasmic 4; T-cell transcription factor NFAT3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NFAT3 |
|---|---|
| Alternative Names | NFATC4 NFAT3 Nuclear factor of activated T-cells cytoplasmic 4 T-cell transcription factor NFAT3 |
| Molecular Weight(MW) | 95449 Da |
| Function | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL- 4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons |
| Tissue Specificity | Highly expressed in placenta, lung, kidney, testis and ovary. Weakly expressed in spleen and thymus. Not expressed in peripheral blood lymphocytes. Detected in hippocampus |
| Cellular Localization | Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals The subcellular localization of NFATC plays a key role in the regulation of gene transcription |
| Entrez Gene | 4776 |
|---|---|
| Nucleotide Accession | NP_004545.2 NP_001129494.1 |
Application
NFATC4 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NFATC4 antibody detected the NFATC4 protein (arrow).
Application
Fluorescent confocal image of U251 cell stained with NFATC4 Antibody (C-term)(Cat#AP13162b).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFATC4 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFATC4 immunoreactivity is localized to Cytoplasm significantly.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
