Application
A. Western blot analysis on (A) HeLa (B) HL-60 and (C) Jurkat cell lysate using anti-Nicastrin RabMAb (Cat. #AJ1544a), dilution 1:1000.| Product Name | Nicastrin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR2575Y |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA0253 |
|---|---|
| Alternative Names | NCSTN KIAA0253 Nicastrin |
| Molecular Weight(MW) | 78411 Da |
| Function | Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Entrez Gene | 23385 |
|---|
Application
A. Western blot analysis on (A) HeLa (B) HL-60 and (C) Jurkat cell lysate using anti-Nicastrin RabMAb (Cat. #AJ1544a), dilution 1:1000.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
