Application
Western blot analysis of NUP50 (arrow) using NUP50 Antibody (Center) (Cat# AP1913a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with NUP50 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of widr cells using NUP50 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NUP50 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NPAP60L |
|---|---|
| Alternative Names | NUP50 NPAP60L Nuclear pore complex protein Nup50 50 kDa nucleoporin Nuclear pore-associated protein 60 kDa-like Nucleoporin Nup50 |
| Molecular Weight(MW) | 50144 Da |
| Function | Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin- alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B |
| Tissue Specificity | Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver |
| Cellular Localization | Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side (By similarity). Note=Localizes to the nucleoplasmic fibrils of the nuclear pore complex (By similarity) |
Application
Western blot analysis of NUP50 (arrow) using NUP50 Antibody (Center) (Cat# AP1913a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUP50 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with NUP50 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of widr cells using NUP50 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
