Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ORAI1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of ORAI1 Antibody (Center) in A375 cell line and mouse testis tissue lysates (35ug/lane). ORAI1 (arrow) was detected using the purified Pab.| Product Name | ORAI1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ORAI1; CRACM1; TMEM142A; Calcium release-activated calcium channel protein 1; Protein orai-1; Transmembrane protein 142A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:50~100 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CRACM1 TMEM142A |
|---|---|
| Alternative Names | ORAI1 CRACM1 TMEM142A Calcium release-activated calcium channel protein 1 Protein orai-1 Transmembrane protein 142A |
| Molecular Weight(MW) | 32668 Da |
| Function | Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1 CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT |
| Tissue Specificity | This ORAI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152~181 amino acids from the Center region of human ORAI1. |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Note=Isoform beta is more mobile in the plasma membrane |
| Entrez Gene | 84876 |
|---|
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ORAI1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of ORAI1 Antibody (Center) in A375 cell line and mouse testis tissue lysates (35ug/lane). ORAI1 (arrow) was detected using the purified Pab.| Application Notes | IHC:1:50~100 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
