Application
The anti-p100 Pab is used in Western blot to detect p100 in placenta lysate.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | P100 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TPX2; C20orf1; C20orf2; DIL2; HCA519; Targeting protein for Xklp2; Differentially expressed in cancerous and non-cancerous lung cells 2; Hepatocellular carcinoma-associated antigen 519; Protein fls353; Restricted expression proliferation-associated protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C20orf1 C20orf2 DIL2 HCA519 |
|---|---|
| Alternative Names | TPX2 C20orf1 C20orf2 DIL2 HCA519 Targeting protein for Xklp2 Differentially expressed in cancerous and non-cancerous lung cells 2 Hepatocellular carcinoma-associated antigen 519 Protein fls353 Restricted expression proliferation-associated protein |
| Molecular Weight(MW) | 85653 Da |
| Function | Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation |
| Tissue Specificity | Expressed in lung carcinoma cell lines but not in normal lung tissues |
| Cellular Localization | Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules |
| Entrez Gene | 22974 |
|---|
Application
The anti-p100 Pab is used in Western blot to detect p100 in placenta lysate.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
