Application
A. Western blot analysis on 293T cell lysate using anti-Phospho-p70 S6 Kinase (pT421/pS424) RabMAb (Cat. #AJ1577c); dilution 1:2,500. Cell lysates were either (1) untreated or (2) treated with EGF.| Product Name | p70-S6 Antibody Phospho-pT421/pS424 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RPS6KB1, STK14A, Ribosomal protein S6 kinase beta-1, 70 kDa ribosomal protein S6 kinase 1;p70 S6 kinase alpha |
| Modification | p-T421/p-S424 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E135 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | STK14A |
|---|---|
| Alternative Names | RPS6KB1 STK14A Ribosomal protein S6 kinase beta-1 70 kDa ribosomal protein S6 kinase 1 p70 S6 kinase alpha |
| Molecular Weight(MW) | 59140 Da |
| Function | Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at 'Thr-412', which is proposed to be a negative feedback mechanism for the RPS6KB1 anti- apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1- 2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Cell junction, synapse, synaptosome (By similarity). Mitochondrion outer membrane. Mitochondrion Note=Colocalizes with URI1 at mitochondrion Isoform Alpha II: Cytoplasm. |
| Entrez Gene | 6198 |
|---|
Application
A. Western blot analysis on 293T cell lysate using anti-Phospho-p70 S6 Kinase (pT421/pS424) RabMAb (Cat. #AJ1577c); dilution 1:2,500. Cell lysates were either (1) untreated or (2) treated with EGF.| Application Notes | WB:1:2500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
