Application
PALB2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PALB2 antibody detected the PALB2 protein (arrow).Application
PALB2 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PALB2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PALB2; FANCN; Partner and localizer of BRCA2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | FANCN |
|---|---|
| Alternative Names | PALB2 FANCN Partner and localizer of BRCA2 |
| Molecular Weight(MW) | 131295 Da |
| Function | Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures |
| Tissue Specificity | This PALB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 553-582 amino acids from the Central region of human PALB2. |
| Cellular Localization | Nucleus. Note=Colocalizes with BRCA2 in nuclear foci |
| Entrez Gene | 79728 |
|---|---|
| Nucleotide Accession | NP_078951.2 |
Application
PALB2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PALB2 antibody detected the PALB2 protein (arrow).
Application
PALB2 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
