Application
PAP Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PAP antibody detected the PAP protein (arrow).Application
PAP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | PAP Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAPOLA; PAP; Poly(A) polymerase alpha; Polynucleotide adenylyltransferase alpha |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PAP |
|---|---|
| Alternative Names | PAPOLA PAP Poly(A) polymerase alpha Polynucleotide adenylyltransferase alpha |
| Molecular Weight(MW) | 82843 Da |
| Function | Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus |
| Tissue Specificity | This PAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-443 amino acids from the Central region of human PAP. |
| Cellular Localization | Cytoplasm. Nucleus. Note=The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic |
| Entrez Gene | 10914 |
|---|---|
| Nucleotide Accession | NP_116021.2 |
Application
PAP Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PAP antibody detected the PAP protein (arrow).
Application
PAP Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
